About fit libraries

Fit libraries are lists of possible positions (non-redundant fits) of your input structures in the EM map. The positions are used in the 1. Global optimization step to generate a large number of combinations of the fits through a Monte Carlo integrative modelling procedure. Each position (fit) has its associated score and p-value, which are used as EM restraints during the modelling.

The fit libraries are typically generated by fitting every input structure into the EM map separately, a procedure handled by the Efitter pipeline of Assembline.

Note

This part of the Assembline pipeline (efitter) will generate all requested libraries per input structure in a single run (automated) according to user’s predefined parameters (see Set up, and following figure).